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small rna cdna library  (Gnomegen Inc)

 
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    Gnomegen Inc small rna cdna library
    Small Rna Cdna Library, supplied by Gnomegen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/small+rna+cdna+library/small+rna+cdna+library/pmc11808964-45-11-22
    Average 90 stars, based on 1 article reviews
    small rna cdna library - by Bioz Stars, 2026-10
    90/100 stars

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    cDNA Library Assay:

    Article Title: Dynamic and functional analyses of exosomal miRNAs regulating cellular microenvironment of ovarian cancer cells
    Article Snippet: .. Total RNAs (3 μg) from each sample were used to generate small RNA cDNA library with a Balancer NGS Library Preparation kit (Gnomegen, San Diego, CA, USA), according to the manufacturer’s protocol. ..

    Next-Generation Sequencing:

    Article Title: Dynamic and functional analyses of exosomal miRNAs regulating cellular microenvironment of ovarian cancer cells
    Article Snippet: .. Total RNAs (3 μg) from each sample were used to generate small RNA cDNA library with a Balancer NGS Library Preparation kit (Gnomegen, San Diego, CA, USA), according to the manufacturer’s protocol. ..



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    Fig. 4. Properties of RnPV18- and RnPV19-derived small RNAs in three different host organisms. (A and B) Size distribution of RnPV18 dsRNA1-(A) and RnPV19 dsRNA1-derived siRNAs (B) produced in plant, fungal, and insect cells. Comparison of siRNAs (sense and antisense, 15 to 32 nucleotides) derived from the RdRP- coding dsRNA1 genomic segments in N. benthamiana cells (Top row), insect S2 cells (Middle row), and the original host R. necatrix W442 mycelia (Bottom row) in each panel. N. benthamiana and fruit fly S2 cells were harvested at 3 d posttransfection for high through-put small RNA sequencing, while R. necatrix W442 mycelia were collected after culturing in potato dextrose broth for 1 wk. Read numbers were normalized against one million small RNA reads. (C and D) Hot spots of RnPV18 dsRNA1-(C) and RnPV19 dsRNA1-derived small RNAs (D) produced in N. benthamiana (Nb), fruit fly S2 (S2) and R. necatrix (Rn) cells. Small RNA reads are mapped to their genomes. The vsiRNA reads were not normalized. (C and F) Ratios in percentage of the 5′-terminal nucleotides of RnPV18 dsRNA1- (E) and RnPV19 dsRNA1-derived siRNAs (F) are shown in graph form. See SI Appendix, Fig. S9, for the properties of the CP-coding genomic segment (dsRNA2).

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Replication of single viruses across the kingdoms, Fungi, Plantae, and Animalia.

    doi: 10.1073/pnas.2318150121

    Figure Lengend Snippet: Fig. 4. Properties of RnPV18- and RnPV19-derived small RNAs in three different host organisms. (A and B) Size distribution of RnPV18 dsRNA1-(A) and RnPV19 dsRNA1-derived siRNAs (B) produced in plant, fungal, and insect cells. Comparison of siRNAs (sense and antisense, 15 to 32 nucleotides) derived from the RdRP- coding dsRNA1 genomic segments in N. benthamiana cells (Top row), insect S2 cells (Middle row), and the original host R. necatrix W442 mycelia (Bottom row) in each panel. N. benthamiana and fruit fly S2 cells were harvested at 3 d posttransfection for high through-put small RNA sequencing, while R. necatrix W442 mycelia were collected after culturing in potato dextrose broth for 1 wk. Read numbers were normalized against one million small RNA reads. (C and D) Hot spots of RnPV18 dsRNA1-(C) and RnPV19 dsRNA1-derived small RNAs (D) produced in N. benthamiana (Nb), fruit fly S2 (S2) and R. necatrix (Rn) cells. Small RNA reads are mapped to their genomes. The vsiRNA reads were not normalized. (C and F) Ratios in percentage of the 5′-terminal nucleotides of RnPV18 dsRNA1- (E) and RnPV19 dsRNA1-derived siRNAs (F) are shown in graph form. See SI Appendix, Fig. S9, for the properties of the CP-coding genomic segment (dsRNA2).

    Article Snippet: Small RNA cDNA library construction and subsequent deep RNA sequencing were conducted by Macrogen Inc. (Tokyo, Japan) with the Illumina platform (HiSeq 2000; 50- bp single- ends reads).

    Techniques: Derivative Assay, Produced, Comparison, RNA Sequencing